Cambridge (CIE) IGCSE Biology
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(Photosynthesis)
Investigating the Rate of Photosynthesis
Investigating the Rate of Photosynthesis: How Fast Can Plants Make Food?
Photosynthesis is the process plants use to make their food, and its rate can change based on light, temperature, and carbon dioxide levels.
What is the Rate of Photosynthesis?
The rate of photosynthesis is how quickly plants produce glucose and oxygen. Scientists measure this rate by observing:
- Oxygen production (e.g., bubbles from aquatic plants).
- uptake (e.g., using a pH indicator).
Key Factors Affecting Photosynthesis
-
Light Intensity:
- More light = faster photosynthesis (up to a point).
- No light = no photosynthesis.
-
Carbon Dioxide Concentration:
- More = faster photosynthesis (up to a point).
- Low slows the rate.
-
Temperature:
- Enzymes involved in photosynthesis work fastest at an optimum temperature.
- Too cold = slow reactions. Too hot = enzymes denature.
How to Investigate the Rate of Photosynthesis
Equipment Needed:
- Aquatic plant (e.g., Elodea or Cabomba)
- Beaker of water
- Sodium bicarbonate (for )
- Lamp (light source)
- Ruler (to measure light distance)
- Stopwatch
Method:
1. Measure Oxygen Production
- Place the aquatic plant in a beaker filled with water and a pinch of sodium bicarbonate (to ensure availibility)
- Position the plant under a lamp and measure the distance between the lamp and the beaker.
- Start the stopwatch and count the number of oxygen bubbles produced in 1 minute.
2. Test Light Intensity
- Repeat the experiment by moving the lamp to different distances (e.g., 10 cm, 20 cm, 30 cm).
- Record the bubble count for each distance.
3. Test Concentration
- Add different amounts of sodium bicarbonate to the water (e.g., 1g, 2g, 3g).
- Count the bubbles for each level of
4. Test Temperature
- Place the beaker in water baths at different temperatures (e.g., 10°C, 20°C, 30°C, 40°C).
- Measure the bubbles at each temperature.
Expected Results
-
Light Intensity:
- Closer light = more bubbles (faster photosynthesis).
- Beyond a certain point, increasing light intensity won’t increase the rate (other factors become limiting).
-
CO₂ Concentration:
- More CO₂ = more bubbles.
- Beyond a certain point, increasing won’t increase the rate.
-
Temperature:
- Photosynthesis increases as temperature rises to the optimum temperature.
- Beyond this, the rate decreases as enzymes denature.
Tuity Tip
Hover me!
Control Your Variables: Change one factor (light, , or temperature) at a time for accurate results.
Optimum is Key: Each factor has a “sweet spot” where photosynthesis is fastest.
Bubbles = Oxygen: More bubbles = faster photosynthesis!
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