Cambridge (CIE) IGCSE Biology

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(Photosynthesis)

Investigating the Rate of Photosynthesis

Investigating the Rate of Photosynthesis: How Fast Can Plants Make Food?

Photosynthesis is the process plants use to make their food, and its rate can change based on light, temperature, and carbon dioxide (CO2)(CO_2) levels.

 

What is the Rate of Photosynthesis?

The rate of photosynthesis is how quickly plants produce glucose and oxygen. Scientists measure this rate by observing:

  • Oxygen production (e.g., bubbles from aquatic plants).
  • CO2CO_2 uptake (e.g., using a pH indicator).

 

 

Key Factors Affecting Photosynthesis

  1. Light Intensity:

    • More light = faster photosynthesis (up to a point).
    • No light = no photosynthesis.
  2. Carbon Dioxide Concentration:

    • More CO2CO_2 = faster photosynthesis (up to a point).
    • Low CO2CO_2 slows the rate.
  3. Temperature:

    • Enzymes involved in photosynthesis work fastest at an optimum temperature.
    • Too cold = slow reactions. Too hot = enzymes denature.

 

 

How to Investigate the Rate of Photosynthesis

Equipment Needed:

  • Aquatic plant (e.g., Elodea or Cabomba)
  • Beaker of water
  • Sodium bicarbonate (for CO2CO_2)
  • Lamp (light source)
  • Ruler (to measure light distance)
  • Stopwatch

 

 

Method:

1. Measure Oxygen Production

  • Place the aquatic plant in a beaker filled with water and a pinch of sodium bicarbonate (to ensure CO2CO_2 availibility) 
  • Position the plant under a lamp and measure the distance between the lamp and the beaker.
  • Start the stopwatch and count the number of oxygen bubbles produced in 1 minute.

 

2. Test Light Intensity

  • Repeat the experiment by moving the lamp to different distances (e.g., 10 cm, 20 cm, 30 cm).
  • Record the bubble count for each distance.

 

3. Test CO2CO_2 Concentration

  • Add different amounts of sodium bicarbonate to the water (e.g., 1g, 2g, 3g).
  • Count the bubbles for each level of CO2CO_2

 

4. Test Temperature

  • Place the beaker in water baths at different temperatures (e.g., 10°C, 20°C, 30°C, 40°C).
  • Measure the bubbles at each temperature.

 

 

Expected Results

  1. Light Intensity:

    • Closer light = more bubbles (faster photosynthesis).
    • Beyond a certain point, increasing light intensity won’t increase the rate (other factors become limiting).
  2. CO₂ Concentration:

    • More CO₂ = more bubbles.
    • Beyond a certain point, increasing CO2CO_2 won’t increase the rate.
  3. Temperature:

    • Photosynthesis increases as temperature rises to the optimum temperature.
    • Beyond this, the rate decreases as enzymes denature.

 

 

 

Tuity Tip

Hover me!

Control Your Variables: Change one factor (light, CO2CO_2, or temperature) at a time for accurate results.

Optimum is Key: Each factor has a “sweet spot” where photosynthesis is fastest.

Bubbles = Oxygen: More bubbles = faster photosynthesis!

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